Journal: NPJ Science of Food

Article Title: Haematococcus pluvialis ameliorates renal fibrosis by restoring mitophagy via PINK1-Parkin-p62-LC3 signaling

doi: 10.1038/s41538-025-00654-x

Figure Lengend Snippet: A Blue: Nuclei. Red: E-cadherin/N-cadherin. Green: Vimentin. IF staining was performed to detect E-cadherin, N-cadherin and Vimentin protein levels (E-cadherin magnification: 100×, scale bar: 15 μm; N-cadherin/Vimentin: magnification: 60×, scale bar: 20 μm). B Quantitative analysis of fluorescence intensity was performed using ImageJ software ( n = 6). C Western blot analysis of E-cadherin and N-cadherin expression in renal cortical tissues from different experimental groups. Quantification of gray values for Western blotting results in ( D ) was performed using ImageJ ( n = 3). α-Tubulin was used as a reference protein. D qRT-PCR analysis of Cdh1, Cdh2 and Vim in different treatment groups ( n = 3). Actb was used as a reference gene. E Functional enrichment analysis of CDH1 , CDH2 and VIM gene via RNA-seq profiling.* p < 0.05 vs. Con/Sham group, # p < 0.05 vs. TGF-β1/UUO group.

Article Snippet: LC3 Polyclonal antibody (14600-1-AP), p62 Polyclonal antibody (80294-1-RR), PINK1 Polyclonal antibody (23274-1-AP), Parkin Polyclonal antibody (14060-1-AP), E-cadherin Polyclonal antibody (20874-1-AP), N-cadherin Polyclonal antibody (No.: 22018-1-AP), Vimentin Polyclonal antibody (10366-1-AP), COL4A2 Polyclonal antibody (55131-1-AP), FN1 Monoclonal antibody (66042-1-Ig), β-Actin Polyclonal antibody (20536-1-AP), and α-Tubulin Polyclonal antibody (11224-AP) were from Proteintech group Co., Ltd (Wuhan, China).

Techniques: Staining, Fluorescence, Software, Western Blot, Expressing, Quantitative RT-PCR, Functional Assay, RNA Sequencing

Journal: NPJ Science of Food

Article Title: Haematococcus pluvialis ameliorates renal fibrosis by restoring mitophagy via PINK1-Parkin-p62-LC3 signaling

doi: 10.1038/s41538-025-00654-x

Figure Lengend Snippet: A Blue: Nuclei. Purple: Mitochondrial. Red: PINK1/Parkin/p62/LC3B. IF staining was performed to detect PINK1, Parkin, p62, and LC3B protein levels (magnification: 100×, scale bar: 15 μm). B Colocalization analysis of p62, LC3B, and mitochondria was performed using ImageJ software. C Colocalization Pearson correlation coefficient analysis were obtained based on IF images of p62 and LC3B ( n = 6). D Western blot analysis of PINK1 and LC3B expression in renal cortical tissues from different experimental groups. Quantification of gray values for Western blotting results in ( D ) was performed using ImageJ ( n = 3). E Blue: Nuclei. Red: E-cadherin/N-cadherin/Vimentin. IF assay was performed to detect E-cadherin, N-cadherin and Vimentin protein levels (magnification: 100×, scale bar: 15 μm). F Quantitative analysis of fluorescence intensity using ImageJ software ( n = 6). G ELISA quantification of CTGF and PDGF secretion in HK-2 cells ( n = 3). H Western blot analysis of E-cadherin, N-cadherin and Vimentin expression in TGF-β1-induced HK-2 cells. Quantification of gray values for Western blotting results in ( H ) was performed using ImageJ ( n = 3). I Representative flow cytometry plots showing the detection of MMP. J Quantitative analysis of flow cytometry fluorescence intensity of ROS ( n = 3). * p < 0.05 vs. Con group, # p < 0.05 vs. TGF-β1 group. a p < 0.05 vs. HP group, b p < 0.05 vs. AST group.

Article Snippet: LC3 Polyclonal antibody (14600-1-AP), p62 Polyclonal antibody (80294-1-RR), PINK1 Polyclonal antibody (23274-1-AP), Parkin Polyclonal antibody (14060-1-AP), E-cadherin Polyclonal antibody (20874-1-AP), N-cadherin Polyclonal antibody (No.: 22018-1-AP), Vimentin Polyclonal antibody (10366-1-AP), COL4A2 Polyclonal antibody (55131-1-AP), FN1 Monoclonal antibody (66042-1-Ig), β-Actin Polyclonal antibody (20536-1-AP), and α-Tubulin Polyclonal antibody (11224-AP) were from Proteintech group Co., Ltd (Wuhan, China).

Techniques: Staining, Software, Western Blot, Expressing, Fluorescence, Enzyme-linked Immunosorbent Assay, Flow Cytometry